FAQs
Frequently asked questions
What is the advantage of Nuvonis Vero cells compared to other sources?
We provide not only Vero cells, but fully tested GMP banks of Vero cells that were generated under serum-free conditions. These state-of-the-art banks can be used to immediately move into GMP production for clinical material, thus speeding up your. There is no need to produce your own cell bank, therefore timelines and project risks can be minimized.
Which viruses can be grown of Vero cells?
As Vero cells have a defect in the interferon pathway, they are a versatile substrate for virus and vector production. Examples include Polio, Smallpox, Rabies, Japanese Encephalitis, Rotavirus, Vaccinia and Influenza vaccines, Vaccinia, Ross River, West Nile, Chikungunya, Tick-borne encephalitis virus (TBE), Hepatitis A, SARS-Coronavirus including SARS CoV-2 etc. After a standard material transfer agreement has been signed, you can immediately test growth of your specific virus on Nuvonis Vero cells.
Do Vero cells grow well under serum-free conditions?
Yes, with doubling times and cell densities comparable to standard Vero cells grown with serum. A major advantage is that both Master and Working Cell Banks were generated under serum-free conditions. In many other setups master cell banks contain serum, which means that every time a serum-free Working Cell Bank is generated the involved adaptation process may lead to changes in the quality of the cells (higher biological variability).
What is the passage level of Nuvonis Vero cell banks?
The Vero GMP-Working Cell Bank is passage 145, the GMP-Master Cell Bank is passage 141. The End of Production Cell Bank (EPCB) was tested at passage 160, thus allowing production on a large scale.
Where do Nuvonis Vero cells come from?
They originate from WHO cells accepted for vaccine production.
Are the Nuvonis Vero cells tested for tumorigenicity ?
Yes, the cells are safe, and an End of Production Cell Bank (EPCB) was tested in a 16-Week Tumorigenicity Assay in Adult Nude Mice (GLP). Full test reports of all quality relevant tests performed according to US FDA, European Pharmacopoeia and ICH guidelines are available on a data site.
What is the advantage on Nuvonis avian cells?
Few avian cells are available on the market. Nuvonis avian cells from quail grow well both in suspension and adherent (single use plastics, microcarriers), making them an ideal substrate for different production processes. We have shown that they can successfully replace Chicken Embryo Fibroblasts (CEF) or embryonated hen’s eggs in production processes.
Which viruses can be grown Nuvonis avian cells?
Examples include Vaccinia, Newcastle Disease Virus, Orthomyxoviridae, HSV Type I, Pseudorabies, Reovirus, Parainfluenza and MVA etc. After a standard Material Transfer Agreement (MTA) has been signed, you can immediately test growth of your specific virus on Nuvonis’ avian cells.
Which medium do I need for Nuvonis avian cells?
The serum-free/animal component-free medium for Nuvonis avian cells is commercially available, please contact us for more details.
Are Nuvonis avian/quail cell banks available in GMP quality?
Yes, the suspension line CCX.E10 is available as fully characterized GMP Working and Master Cell Bank.